Is anybody in Sweden interested for collaborative Indo-Sweden Research Project?

Warm Greetings! I'm glad to bring to all of your notice that there is a call for collaborative research project funding from the Department of Science and Technology, India entitled "INDIA-SWEDEN...

04 August 2019 2,049 8 View

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23 May 2019 3,584 10 View

Why non-technical jobs are more paid at the beginning???

In recent past it has been observed that in most of the cases non-technical peoples are actually earning more than the white collar roles at the beginning of their career in different farms. Is it...

16 April 2019 5,664 19 View

Do you think your physical fitness can impact your research capabilities?

Being healthy is really important and I do believe staying physically fit can impact your research in a positive way due to the direct correlation of our mental physical wellness. Please share...

03 April 2019 4,669 86 View

Chitosan coated with alginate?

I am wondering if is it possible to firstly encapsulate bacteria with chitosan and then coat them within alginate-beads? Can anybody provide me with the procedure? Thank you

08 November 2018 6,625 3 View

Do I need to remove microbeads before putting CD31+ positively selected cells in culture?

I am isolating cells by CD31+ microbeads using magnet?

18 December 2015 9,805 3 View

Could anyone help with why the protein (Myc-DDK tagged) not expressing in HEK293T cells?

I am working on a protein which is encoded by 6.3kb of cDNA. The expected molecular weight of the protein is 236kDa. I have the commercially purchased cDNA clone of the same in a Myc-DDK backbone...

20 August 2014 2,528 5 View

Is there any software for predicting disulphide bonds in a protein?

I want to see the possible disulphide bond formations in a particular protein.

23 October 2013 2,572 18 View

What are the essential criteria for designing a probe for northern blot?

What should be the minimum probe length, the GC content and annealing temperature? Is a DNA or RNA probe more preferable? Is there any software to help design probes or can I just pick up a...

12 August 2013 8,113 6 View

What are the best possible softwares for ORF prediction?

Prediction softwares especially with respect to structure and function

01 October 2012 4,435 9 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View