If a bacterium grows vigorously forming bit slimy situation in N free agar plates , is it nitrogen fixing?
For publishing transformation related method I need name of some journals without costing us Article Processing Charges. Kindly help.
03 May 2024 1,896 1 View
We are purifying the protein from recombinant DNA
05 January 2024 1,086 2 View
I am actively seeking an opportunity to contribute a chapter entitled "Environmental Health Hazards Attributed to Deteriorated Indoor Air Quality Caused by Inferior Construction Practices" to an...
06 November 2023 1,584 1 View
What could be possible reason for a bacterium not to grow in N free broth (kept at 28 degree in shaker incubator) but grows on the same agar media on plate (kept at 28 degree)? Is it because the...
24 May 2023 4,489 0 View
How often does this happen in the laboratory?
21 February 2023 4,154 4 View
I am facing a MACH1 transformation related problem. My plasmid is PCMU-GAr which contains spectinomycin resistant gene. I've transformed the same plasmid in MACH1 and am getting the lawn of MACH1...
06 July 2022 4,319 4 View
I have read a few papers on this topic but the simulation procedure hasn't been mentioned anywhere. Only results have been shown.
28 October 2021 3,380 4 View
Dear all, I would like to know how to combine two or more DNA barcode sequences in one phylogenetic tree. Do you combine all sequences first to make one long stretch of sequence and align it with...
17 October 2021 6,325 4 View
Can anybody explain why at one retention time give several hits? Then which compound to choose from the hits? Thank you
06 May 2020 2,513 0 View
Dear All, is it allowed by WHO to use alcohol from fermentation for hand sanitizer or for sanitation, even the alcohol level reaches 80%? Thank you. Best regards.
19 March 2020 2,703 9 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...
05 August 2024 5,311 0 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
I am doing a study on nursing students satisfaction on the hospital attachment whether they are satisfied with the clinical attachment or not. I need more research studies on this
03 August 2024 6,985 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
for eg. transition shock scale
01 August 2024 5,998 0 View
Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.
01 August 2024 10,046 1 View