I am doing SNP genotyping on Lightcycler 480 using taqman probes and Taqman Universal Master Mix with UNG. Sometime the software get a low signal for heterozygous samples and the software is not able to call it as "both alleles". Will there be any differences if I include the UNG activation step in my protocol? Is there any way to increase the heterozygous call of FAM and vic?