Abhinav Chaudhary

9 Questions 22 Answers 0 Followers

Questions related from Abhinav Chaudhary

DNA extraction from cotton lint?

Hi, Can anyone tell if there is any published method for isolation of genomic DNA from cotton lint fibres. If not, can anyone give an insight for any work being done anywhere in the world on...

17 February 2016 9,127 1 View

Why does one particular primer not amplify in FFPE extracted DNA?

I have tried isolation of DNA from FFPE tissue using a variety of methods inclding qiagen kits and manual ctab method, with and without using Xylene. etc. I am trying to amplify a few cancer genes...

15 October 2014 5,020 3 View

Any advice on Thalassemia Major or Minor offspring?

I am learning about the hereditary genetics of Thalassemia. It is divided into two classifications, beta-plus and beta-zero; and a thal-major has either one of the two homozygous states. Now there...

14 December 2013 8,087 4 View

Any suggestions for genetic risk prediction?

I am trying to understand the implication of some SNPs responsible for disease in cattle. I want to calculate and predict the risk of disease from risk allele of an SNP in an individual animal...

29 November 2013 2,775 1 View

Do I need to activate UNG in Taqman Universal Master mix?

I am doing SNP genotyping on Lightcycler 480 using taqman probes and Taqman Universal Master Mix with UNG. Sometime the software get a low signal for heterozygous samples and the software is not...

08 November 2013 3,471 4 View

TaqMan SNP genotyping: Is there a need to use the UNG activation step?

I am using TaqMan probes for SNP genotyping on LightCycler 480. I am facing some difficulties in resolution of heterozygous genotypes. The software can not identify it as " both alleles"...

07 November 2013 7,576 2 View

TB detection kit for Roche Light Cycler

Can anyone please suggest a kit for Mycobacterium tuberculosis detection on Roche Light Cycler 480 II?

26 April 2013 1,012 1 View

Blood parasite detection using PCR?

Can anyone tell me how long it is good to store animal blood without reducing the parasite detecting efficiency? I tried isolating whole blood DNA after 2 months of storage and it is not giving...

21 February 2013 4,923 2 View

Does anyone know a way to remove genomic DNA contamination from Mitochondrial pellet using DNAse treatment?

I am facing the problem of genomic DNA contamination in my mitochondrial DNA isolation from insect tissue. I want to include a DNAse treatment of my whole mitochondrial pellet before proceeding...

21 December 2012 9,312 6 View