So I want to carry out antiinflamatory studies using cell lines in which I treat the cells with LPS and then check for IL-1 beta, NO, PGE2 etc. My question is do I need to use specific cell lines for this? I have seen people use RAW 264.7, HL-60 and THL-1 cells but I do not have these cell lines at the moment. Can I use just any other cancerous and non cancerous mammalian cells?
Hi, the in vitro anti-inflammatory activity can be evaluated using RAW 264.7 cells stimulated with LPS and/or INF-γ.
Yes saw literature that used RAW 264.7 cells.....but I do not have these cell lines and can't get them any time soon
The simple answer is no, you just need a cell line or a primary cell the express TLR-4, and the most common cells used for LPS studies are macrophages. A more specific answer can be more complicated depending on the goals of your study.
Also... I'm pretty sure ALL cell lines are "cancerous" hence they are "immortal".
I agree with Shen-An Hwang absolutely. You need a macrophage cell line for any in vitro antiinflammatory study because they are phagocytic cells capable of stimulating the synthesis of inflammatory cytokines and other inflammatory molecules.
Yes, you can use either cancerous or any other cell line for your study. But, just make sure that your cells do express the inflammatory/anti-inflammatory signalling molecules that you are interested in.
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