Do I need glycerine to weigh down samples of DNA/ Protein?

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What additional measures are suggested based on the study's findings?

In the study entitled "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis" by Zhenzhen Pan, et,al. (2023), what additional...

11 April 2024 5,964 0 View

What barriers exist in recruiting voluntary blood donors in Western Kenya, and how can these be addressed to improve their blood bank operations?

According to Kavulavu et al. (2022) in their research article "Challenges facing blood transfusion services at a regional blood transfusion center in Western Kenya", they identified understaffing,...

11 April 2024 2,344 0 View

How can I simulate VANET using OSM, SUMO and NS2, specifying WiMAX as the protocol/technology for the V2R communication and IEEE 802.11p for the V2V?

I am new to these network simulators (OSM, SUMO and NS2), but need to use the simulators for a project i am currently working on. i am total a beginner at these simulators, therefore, i will...

28 August 2020 2,981 5 View

Can I use 30% sucrose step when staining with PAS (glycogen detection in tissues)? As its a small sugar will this generate a false positive?

I wish to look at glycogen accumulation in cardiac tissue using PAS staining on frozen sections of tissue. I am using formalin fixative, followed by a sucrose gradient until the tissue sinks, then...

28 May 2019 4,757 1 View

Is artisanal tin mining a profitable activity in Jos?

What is the average income of artisanal miners from this activity

23 April 2019 3,310 4 View

Does anyone know if there is a laboratory in the Philippines that offers to examine animal mucin in order to know its components?

Does anyone know if there is a laboratory in the Philippines that offers services to examine animal (preferably snail) mucin in order to know its components?

31 August 2018 8,145 0 View

Are there survival estimates for birds from East/Southeast Asia?

I am looking for estimates of apparent annual survival for birds from anywhere in Asia, such as those produced from mark-recapture data collected from bird banding or ringing stations. I know...

26 August 2018 2,967 1 View

What is a good architecture thesis project under the theme of 'Megacity'?

I am an architecture student in the Philippines and I am currently doing some initial research for my thesis project under the theme of 'Megacity'. I would like to ask for some suggestions on...

11 May 2018 9,336 6 View

Does rushing the editorial and review process undermine the quality of academic publication?

Several journals (I won't specify) appear to be responding to a need for accelerating the editorial and review process for publishing academic papers by drastically reducing review periods (e.g....

11 October 2017 9,553 6 View

Is there an interactive reference source that efficiently maps the elements of Quantum Mechanics?

Given: https://www.ted.com/talks/manuel_lima_a_visual_history_of_human_knowledge?utm_source=facebook.com&utm_medium=social&utm_campaign=tedspread#t-756968 I wonder: Is there a useful...

15 September 2017 2,298 11 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View