I wish to look at glycogen accumulation in cardiac tissue using PAS staining on frozen sections of tissue. I am using formalin fixative, followed by a sucrose gradient until the tissue sinks, then standard freezing, cutting and staining. I am wondering if the use of sucrose may generate false positives as it's a carbohydrate? Is there some merit to this? my histology experience is extreamly limited so any suggestions would be terrific :)