What percentage of IL-17 positive cells are expected to be positive in Th17-polarized cells in flow cytometry?

16 May 2024 0 7K Report

Day-1: Coating anti-CD3 and anti-CD28

Day 0: Seeding of purified CD4 cells + Cytokine cocktail for Th17 polarization (IL-6, IL-23, IL-1beta) + Anti-IL-4 & Anti-IFN-gamma

Day 5: PMA+ Ionomycin stimulation

IL-17 staining

...........................................

How can we differentiate polarization and IL-17 staining issues in case of

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