How to implement monte carlo method to compute state value in matlab ?

assume there are 80 states each with a possibility of 2 actions. we have a predefined reward function. The problem is about research allocation.

30 October 2020 9,930 3 View

Need help for Site directed mutagenesis using Phusion...

20 March 2016 763 9 View

In electrochemical transient techniques for investigating the electrochemical kinetics which yields optimal result, Galvanostatic or Potentiostatic?

In electrochemical transient analysis for investigating the electrochemical kinetics which yields optimal result, Galvanostatic or potentiostatic?

04 February 2016 987 5 View

How can I dissolve methonolic extract in deionised water for in vivo analysis using hepatocarcinoma in vivo SD rat model?

i am planning to dissolve methonolic extract in deionised water but in articles people have mentioned to dissolve their extracts in carboxy methyl cellulose 2%, DMSO 2% and PEG 2%. but i tried to...

28 May 2015 9,765 4 View

Could anyone suggest any crosslinker which will react with Gold nanoparticle treated PMMA substrate for protein detection?

When I coat cysteamine (as crosslinker for biomolecule adsorption) on gold nanoparticle treated PMMA substrate, Gold nanoparticles are desorbed. Could anyone suggest any crosslinker which will...

26 March 2015 3,663 6 View

I have a marine extract and am finding a whitish colored tissue degraded substance in it. What could this be?

I have a marine organism. When I conducted cold extraction for it, I got crude extract for non-polar to polar solvents for around six different solvents. When I tried to dissolve these crude...

24 April 2014 9,788 5 View

Is Volterra space related to preopen sets only? If not, define the other sets...

Weak Volterra space

19 January 2014 8,787 2 View

I want to strip a western blot. Can you suggest an efficient method?

I have stripped with a harsh stripping reagent such as B-mercapto ethanol but I lost most of the protein

24 April 2012 626 18 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View