i am planning to dissolve methonolic extract in deionised water but in articles people have mentioned to dissolve their extracts in carboxy methyl cellulose 2%, DMSO 2% and PEG 2%. but i tried to dissolve my extract in deionised water and its taking time to dissovle completely but its dissolving.so can i use deionised water for hepatocarcinoma invivo SD rat model?? and for invivo SD rats how much mL of deionised dissolved extract can i give orally??
Dear Shankar,
Complete dissolution of extract depends on its chemical components. As it is plant extract, there must be some hydrophilic and some lipophilic compounds. If the extract contains more lipophilic constituents, then some part will dissolve in water and most of the part will remain as it is and vice versa.
And as your sample is water soluble, you should use water, although it takes more time to dissolve. Because other solvent have the possibility to show at least some pharmacological activity where water don't have any possibility to have any activity.
Besides, for animal experiments, our main concern is to administer the drug sample only. So you should administer as lower as possible but the volume should be same for control and treatment groups. For rats the highest volume can administer depends on its body weight (10 ml/kg).
Dear Md Moniruzzaman ,
Thank you for your reply..my main concern is not ordinary water its deionised(without any ions in it and pH is arround 7-7.3 same pH as i used for my invitro study to dissolve extracts in PBS buffer). is it possible to use deionised water. maximum ill go upto 2-3mL..rite now rats are arround 320-360g.
Dear Mr. Sankar,
In previous I meant deionized water. Of course you can use it. Besides, you also can use normal saline (0.9% NaCl). And as your rat body weight is 320-360 g, so you can administer highest 3.3-3.6 ml. But my suggestion is, if you can, try to administer less volume.
Best of luck.
Dear Md Moniruzzaman,
Thanks for your kind advice. ill keep in mind regarding the volume..
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