I have Endothelial Cell Growth Medium, cat no. 211-500, Sigma-Aldrich. For the culture of Human aortic endothelial cells do I have to add FBS, antibiotics and other supplements with this medium which is written "all in one ready to use" in sigma website?
Please suggest.
As per the manufacturers guidelines, you need not to add anything in the medium. the Media is supplemented with all necessary things.
https://www.sigmaaldrich.com/technical-documents/protocols/biology/human-aortic-endothelial-cells.html
Jitendra Kumar Shandilya ya it is written "all in one ready to use" in sigma website . Thank you for your reply.
Hi Dr Rinku Baishya ,
1. Did you procure it at your own, if yes, then you must see the information leaflet provided with product.
2. If you don not have then you have two ways:
a) either run a small experiment in two flask one with FBS and antibiotics and the other without these.
b) you can write to sigma and can ask for details about this particular medium.
Thank you,
Good luck
Dear Dr Rinku Baishya ,
I hope you will share your experience.
How did you come to reach the conclusion?
Thank you
Since it is an all in one ready to use medium, you don`t have to add further supplements.
Deepak K. Goyal ....cells are not adhering. Its already more than 5 days. I have not added anything in the media.
dear Dr Rinku Baishya ,
pls ref. my first reply...............................
good luck
Deepak K. Goyal I have changed one flask with FBS and antibiotics and another one with only ready to use media. But still its not adhering.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Methods
- Cloning