Dr Rinku Baishya

5 Questions 13 Answers 0 Followers

Questions related from Dr Rinku Baishya

Do I have to add FBS, antibiotics and supplements to Endothelial Cell Growth Medium, cat no. 211-500,Sigma-Aldrich for the culture of HAoEC?

I have Endothelial Cell Growth Medium, cat no. 211-500, Sigma-Aldrich. For the culture of Human aortic endothelial cells do I have to add FBS, antibiotics and other supplements with this medium...

21 August 2020 4,565 9 View

MDA-PCa-2b cell culture growth problem

I am using BRFF-HPC1 media for growing MDA-PCa-2b cells. My cells are not growing well and they are growing very very slow.I used 20% FCS and pen-strep in the HPC1 media. There is no glutamine and...

31 May 2017 6,341 3 View

Can anyone tell me whether Pbf group is also removed when Mtt group of lysine is removed?

Whether Pbf group of Arginine is removed when Mtt group of lysine is deprotected with TFA/DCM/TIS (1:94:5). Deprotection of Mtt group was done by 5 minute nitrogen purging with TFA/DCM/TIS (3...

04 December 2014 8,723 5 View

Can anyone help me in the HPLC quantification of a drug?

I am studying the in vitro release study of a drug nanoparticle. When I inject the standard drug solution to HPLC to make a calibration curve, it shows different areas of the peak of the same...

23 October 2014 6,776 4 View

Why am I not getting the coupled peptide after fmoc deprotection and cleavage?

I have coupled Gemcitabine to a cyclic peptide. I got the corrrect mass to the last fmoc protected amino acid. But after fmoc deprotection and cleavage from resin I am not getting the coupled...

05 September 2014 6,862 6 View