I am working on HepG2 cells and trying to establish a toxicity model by monitoring the levels of liver enzymes viz AST, ALT, ALP and GGT. I am getting the release of AST and ALT. How do I make sure it also releases the other two? And should cells be releasing all four when damaged? Or is it case specific?
Sharing any suggestion or practical experience will be greatly appreciated.
I have worked with HepG2 cells for many years. I have used many indicators of toxicity in HepG2 cells with nanomaterial and metal ion exposures. My favorite "released enzyme" biomarker is LDH. AST and ALT are not as responsive as LDH in my experience. The HepG2 cells will release enzymes at different points in the dose -response curve.
Kirk Kitchin PhD DABT, US EPA
Ok yea that's a valuable input. Thank you. Over the period of years, I also switched to data coming from ROS shift for reliability and reproducibility in comparison to these enzymes.
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