Hello, Greetings of the day,
I want to calculate the relative quantum yield w.r.t. quinine sulfate (QS). Before going to sample, I want to ensure my standard (QS) and tried to calculate the relative QY of 2-Aminopyridine (reported QY is ~64%) (both solutions were prepared in 0.1M H2SO4), on calculation, I am not getting the value closer to 64%. After going to the methodology again I encountered with the term 'fully corrected spectra'. Can this be a reason for the incorrect QY of 2-aminopyridine? How to resolve this?
I am following the following manual for the calculation of RQY- https://static.horiba.com/fileadmin/Horiba/Application/Materials/Material_Research/Quantum_Dots/quantumyieldstrad.pdf
Thank You
The particular spectrofluorimeter software will contain a correction file. This is simply a list of correction factors for each wavelength. The correction factors are used to correct for the peculiarities of the instrument for detecting fluorescence at each wavelength. Use the correction feature of the software to correct the spectra with this file.
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