Can publishing a prepublished research as a new book chapter be acceptable in the academic world?

Research papers are in brief, book chapters are in detail. Can publishing a prepublished research as a new expanded book chapter be acceptable in the academic world?

19 March 2020 6,221 9 View

Why does the universe expand despite self-gravity?

We are in an expanding Universe as per the big-bang cosmology. Does it really mean that the outward thermal pressure now beats the inward gravitational pressure in the Universe? What is the exact...

14 March 2020 4,196 37 View

What are the different projects launched with a strong mission to make knowledge globally free?

What are the different projects launched with a strong mission to make knowledge and its resources globally free so that unprivileged and underprivileged scholars could pursue higher education in...

28 December 2019 9,526 3 View

How can we prevent ear-problems often encountered by passengers after a flight journey?

The vertical atmospheric air pressure differences encountered during flight journeys often create ear-problems well realized by passengers after landing. How can we prevent them easily?

26 November 2019 9,015 12 View

What is the significance of dynamical system analysis in cosmology and dark energy study??

What is the reason that we modify our system as dynamical systems while studying dark energy? And further, what is the criteria for choice of the dimensionless variables x,y,z.??

29 September 2019 7,687 1 View

How to get low energy model Hamiltonian of a material from DFT calculation?

I need to find the low energy model Hamiltonian for a material (semimetal). I heard that this is possible by using Wannier90 clubbed with VASP or QE. It would be very helpful for me if someone...

18 September 2019 6,649 2 View

What are the additional advantages that a Fano resonance possesses in comparison to normal dipolar resonance in Optical systems?

Fano resonance is a distinctive spectroscopic behavior in many optical systems. There're certain differences in terms of physics and behavior of Fano resonance compare to dipole resonance which is...

30 May 2019 4,244 2 View

What is the best process for estimating Triclosan from sediment samples?

What is the best and easiest protocol for extracting Triclosan from sediment and further estimation using HPLC, GC-MS or LC-MS? Please suggest some easy and dependable protocol.

15 March 2019 2,007 4 View

Do electrons and protons have independent minds to decide the directionality of gyromagnetic effects?

We have mind. We are made up of matter. Does matter have its own mind? Do electrons and protons, which are the building blocks of matter, have independent minds to decide the directionality of...

25 February 2019 2,405 3 View

Will any expert recruit me as a postdoc under his/her supervision in the field of bioenergy or waste water treatment? Will you please share this?

I, Dr. Rachan Karmakar, have done my Ph.D. in 2018 in the field of energy and environment. My areas of specialization are as follows- 1. Biofuel production and characterization 2. Analysis of...

15 January 2019 9,065 5 View

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03 March 2021 5,360 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View