Best protocol for automated cell counting?

Hi all, I am performing an exosome quantification assay and I need to normalize my results to total number of cells. I will not be able to detach my cells from the plate, and therefore cannot use...

17 November 2020 6,583 2 View

Do I need collagenase to dissociate hepatocytes?

Hi all, after culturing hepatocytes in a monolayer on collagen in 24 well plates for 5 days, I attempted to trypsinize (0.25% trypsin) them to perform a cell count. However, none of my cells...

11 November 2020 2,478 2 View

Can I use an IHC antibody for ICC?

I am planning a double antibody ICC on hepatocytes using polyclonal antibodies; however, one of the primary antibodies I want to use is only verified for use in IHC. Can I adapt it to be used in...

16 August 2020 4,073 3 View

Good protocol for metabolite extraction from clostridia?

I am looking for a reliable metabolite extraction protocol for clostridia. Most commonly acetonitrile/methanol/water (2:2:1, v/v/v) is used, however, some research claims that pure methanol is...

26 February 2019 1,126 2 View

How are Refractive index changes controlled?

When doing UV-Vis spectroscopy, how do you control for changes in refractive index? Isn't that something that the UV-Vis machine does automatically?

09 April 2018 8,581 4 View

Where can I find commercial ion selective membranes?

I know there are ion selective electrodes (ISEs) out there for measuring specific ion concentration and these electrodes have an ion selective membrane within them. Where can I purchase the...

02 November 2017 2,924 9 View

Anyone have a good way to quantify total levels of TRIF (Ticam1) using Western blotting or FACS (in murine macrophages)?

18 February 2016 9,107 1 View

Is anyone conducting research dealing with coaching models or reality TV?

I am interested in two types of research topics: One factors involved with successful coaching models used in couples or marriage coaching. Most coaches use Co-Active coaching, GROW or REGROW....

09 January 2015 8,887 3 View

Is anyone conducting research dealing with coaching models or reality TV?

I am interested in two types of research topics: One factors involved with successful coaching models used in couples or marriage coaching. Most coaches use Co-Active coaching, GROW or REGROW....

31 December 2014 9,531 4 View

Can someone please help me understand some aspects of conducting a factor analysis please?

I have collected my data and I am now analyzing it. Here are the areas I cannot understand: 1. The pre-post tests questions are alike every way (except in how participants respond after exposure...

18 October 2014 9,540 7 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View