I want to study if MMP7 and thrombin are involved in a certain pathway by using blocking antibodies to these two molecules in mouse primary cell cultures. Does anyone know if these are available? anti-human MMP7 (R&D) and thrombin (Novus) antibodies can be used for blocking-but will they work in mouse cells?
Any information is appreciated.
I have a question rather:
Aren't blocking antibodies the one that block your antigen to bind to any other antibody. e.g. some IgG blocks binding of IgE to protect allergic reaction. or Blocking antibodies that protects against embryo rejection
How are blocking antibodies useful in In vitro assay?
And cant u just order a biotech company to screen Anti-human MMP7 and Thrombin blocking antibody for you?
Blocking antibodies are so termed because they bind to the speciic antigen protein and block bindging of this protein to its receptors/ligands and inhibit further downstream signaling and other functions Blocking antibodies are used to determine the functions of proteins.