I am using cBirA mice that are designed to express BirA (biotin ligase) and EGFP following exposure to Cre recombinase. I crossed these mice with my own mice carrying BAP tag. I am wondering if I need to add biotin supplement into their diet to promote the biotinylation of my BAP tagged protein. I will need to use these mice for streptavidin pulldown in future. I am new to mouse works so I would appreciate any advice.

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