During transfection of B cells with plasmids/linear DNA fragments, do the large amounts of foreign (bacterial) DNA sequences activate signalling cascades, like that of TLR9 for CpG-rich sequences (promoter/regulatory regions)? If no, why? If yes, will that not affect the experiment in hand?
Hi Devi,
As you know, often times it is very difficult to achieve high delivery efficiency without compromising the experiment in B cells. May I ask if you have tried Self-Deliverable RNA silencing FANA Molecules to achieve high delivery efficiency in B cells?
If not, I encourage you to review the attached FANA delivery protocol without the use of any transfection reagents.
Please kindly let me know if this can help your specific experiments?
Have a good day!
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