What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

How to isolate the pure Ruthenium DMSO complexes from the reaction mixture?

The reaction between N-S bidentate/ONS tridendate ligand (TSC) and cis[RuCl2(DMSO)4] in ethanol by reflux is complete as seen from TLC chromatography. But removing the excess solvent through...

27 December 2020 6,630 1 View

What may be the reasons for broad spectrum in proton NMR (taken in DMSO-d6) for Pd(II) complexes?

The synthesized Pd(II) complexes involved -NH group attached to C=S moiety. The 1H proton NMR taken in DMSO-d6 solvent consisted of a very broad peak at 4.8 ppm with an integration of 6. They are...

09 December 2020 4,340 3 View

What may be the reasons for broad spectrum at 4.8 ppm in proton NMR (taken in DMSO-d6) for Pd(II) complexes?

The synthesized Pd(II) complexes involved -NH group attached to C=S moiety. The 1H proton NMR taken in DMSO-d6 solvent consisted of a very broad peak at 4.8 ppm with an integration of 6. They are...

09 December 2020 9,960 3 View

How to change the protein chain names X into A, B, C, D after MD simulations in Gromacs?

I am running MD simulations for holoenzyme in Gromacs. The protein has four chains like A, B, C, D. After MD simulations, All the chains are showing as X chain in VMD and even in the PDB file....

12 November 2020 6,228 1 View

How to perform power analysis for logistic regression with an interaction between continuous and binary categorical predictor?

In one of my studies, I need to assess the sample size. The study aims to predict the choice of participants with a continuous predictor and a categorical predictor (control vs. experimental)....

26 October 2020 8,631 6 View

I am electroplating Ni on a stainless steel. At temperature and time should I anneal so that Ni thermally diffuses into stainless steel?

I am electroplating Ni on a stainless steel for abrasive application. At temperature and time can I anneal so that Ni thermally diffuses into stainless steel? My intention is to improve the...

20 October 2020 7,092 3 View

Can anyone help me to get all the variables imported in NLogit?

While i try to import the data from excel files to NLogit 4, only 18-19 variables being imported and the rest 6-7 variables is missing in the imported list. I have tried in changing the memory...

12 November 2019 9,760 1 View

Which breast cancer cell line is good for metastasis study in NOD SCID mice model?

13 June 2018 5,388 3 View

I am working on ISSR primers. I get bands for some samples and a smear in others. Why?

Hi, I am currently working on ISSR primers for my algal samples. When I did PCR for my samples along with some marine algal specimens, I could observe bands for the marine specimens and a smear...

15 May 2018 877 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

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22 February 2021 9,932 11 View