Is this method of modeling plastic behavior in Abaqus correct?

I've to model the plastic behavior (Yield Stress vs Plastic Strain) of a polyimide material (Elas_Mod=2500MPa) in Abaqus. The engineering stress vs strain plot is shown as the 3 red curves. I've...

03 February 2021 7,728 3 View

What are the insilico tools available to recognize transcription binding sites on a promoter ?

I have to control the expression of a gene ( in vitro ). For designing an optimal promoter assay, I would like to know more about Transcription factor binding region, enhancer and repressor...

11 January 2021 7,992 3 View

Can someone tell, how to implement kernel density estimation in images so as to employ spatial bandwidth and range bandwidth separately in matlab?

implement kernel density estimation in images so as to employ spatial bandwidth and range bandwidth separately in Matlab

13 August 2020 1,092 1 View

How we can measure the infiltration in soil having gravels more than 30 % ? Please suggest correct way for measurement of infiltration rate ?

How we can measure the infiltration in soil which having gravels more than 30 % . We want to calculate infiltration rate in our research work. Kindly suggest correct way for calculation of...

27 July 2020 829 3 View

Can anyone share experimental manual of Arabidopsis thaliana book?

I am working on this model system. So I am looking a book on this model system which can help me designing experiments and perform them in my laboratory. I will be thankful for you. thanks

08 June 2020 3,157 2 View

How to perform simultaneously structural (Rietveld like) and microstructural analysis using WPPM method implemented in PM2K software for powder XRD?

I am learning Whole Powder Pattern Modelling (WPPM using PM2K) of powder XRD data to estimating microstructural properties. In this approach peak intensities are treated as free fitting parameters...

30 April 2020 7,802 1 View

How does the preprints are different from published article?

What is the way of publishing preprint and where to publish the biological data in a preprint form? Next how to convert the preprints into a research articles?

09 April 2020 139 3 View

Indicator variable - Pooled regression results vs data partition results?

This is the Base regression: Y = b0*X1+b1*X2+error term I am using an indicator variable Z which takes value 1 for years 4 to 6 and value 0 for years 1 to 3. I am trying to analyse the impact of...

05 March 2020 9,865 3 View

What is importance of dew in crop production and productivity in semi arid region of India ?

I am interested to know importance of dew in crop production and productivity in semi arid of India. How it play important role during tress period.

23 February 2020 974 3 View

What is the alternative method to centrifugation to remove acid residues from sediments during carbonate removal process?

I am removing carbonates from clay and sand samples. So, I treat the sediments with H2O2. I need to wash the sediments to remove acid residues. I am thinking of heating the sediments with...

06 December 2019 5,057 6 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

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28 February 2021 9,936 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View