Does anyone have a working protocol for the extraction of DNA and/or RNA from DBS?
Chelax method used by people working on malaria works best for DNA. We have used a different protocol fdor RTPCR analysis on extracted RNA also (Mlambo et al, 2008, AJTMH).
Qiagen RNeasy Mini Kit worked well for me in extracting RNA from DBS.
Here's a methods paper on extracting DNA from DBS (Ghantous et al., 2014).
Can anyone help me out ? Which PK software is available for free download from internet and is reliable too?
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