We've got an immortalised cell line that was being DNA profiled to be certified as a valid cell line for a culture bank. The profile results showed that this had the profile of a breast carcinoma cell line. Hence we supposed a contamination.
Therefore we took all our cell lines for DNA profiling to avoid further problems. One of them, a trophoblast cell line showed homologies with a pancreatic carcinoma line. When I checked the pancreatic cells profile it had exactly the same profile reported for the trophoblasts.
That makes me doubt from validity of the DNA profile of the first cells.
Has anyone had a similar experience?
Javier,
Are you using a commercially available cell line from ATCC? If so, check out the link below that lists misidentified cell lines.
http://www.atcc.org/CulturesandProducts/CellBiology/MisidentifiedCellLines/tabid/683/Default.aspx
Thanks Sakthikumar
The cells we tried to identify were a new cell line, immortalised with the hTERT method. Unfortunately they are not coming from ATCC.
When they were profiled they showed results for a breast carcinoma line.
That's very strange. Have you checked the original cells that you used to immortalize? I am afraid that you will have to do some police work to trace back to the beginning to ensure that you used the cells that you think you used to immortalize. And make sure that those cells are somehow not contaminated with the breast carcinoma cells that your cells match to.
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