Angular momentum and the Dirac equation - interpretation?

The Dirac equation for a free particle reads H = c*alpha*p + beta*m*c^2 (Sorry for the miserable formatting - how to enter Latex Code or similar?) The commutator [H,L_i] is nonzero for any...

01 February 2021 7,037 1 View

How to measure abundance in an LC-MS/MS experiment?

Is the chromatographic peak a valid measure of a compounds` (relative) abundance in LC-MS data? We have recorded three MS/MS spectra; their retention time falls into a specific chromatographic...

13 January 2021 6,685 6 View

Which collaborative tools for interactively writing R code are out there?

I am searching for tools to collaboratively write R code together (and run it in an online environment). Crucially, the functionality should be similar to Google docs, i.e. all users should be...

26 May 2020 6,846 6 View

What are the greatest insights academic philosophy achieved during the past 100 years?

I'm seeking a justification for the existence of academic philosophical work. My question is about established 'insights': stable advances, which are largely exempted from any further controvery...

25 April 2020 644 9 View

Carbon coating of metal particles?

I am searching for potential project partners (companies) with experience in the carbon coating of metal particles.

03 March 2020 8,208 4 View

Method for the extraction of insect melanin?

Dear colleagues, does anyone know a reliable method for the extraction of melanin (from both cuticle and formed by immune activated phenoloxidase in the body cavity) from whole insects? Thank...

24 May 2019 962 3 View

Does anyone have barnase-resistant E. coli?

Promega is selling those Flexi-System Vectors. However, they come with Barnase as a selection marker and hence cannot simply be transformed into your usual library-competent E. coli strain. Since...

14 January 2019 480 4 View

Can one use 1,5-DAN (97% purity) for MALDI analysis?

1,5-DAN is a fantastic matrix for MALDI ISD (in-souce decay) analysis. Can anyone tell me whether one can use the cheaper 1,5-diaminonaphtalene (97% purity) instead of the much more expensive...

25 November 2018 211 3 View

Detection of pathway activation at the end of an experiment - which methods exist?

Problem: we want to know - at the END of an experiment - if a signalling pathway was activated DURING the experiment. I had read something about a novel technique that allowed this. I think that...

05 November 2018 9,372 3 View

Gaussian16, dG solvation free energy?

Is there any keyword missing to get an idea of the dG solvation free energy of a molecule? # M062X/6-31G* Opt SCRF=(SMD,1stVac,Solvent=water) From what I understand from the gaussian09 user...

25 March 2018 1,196 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View