Hi Rishabh,

If you just wanted to test if DNA contamination might be an issue in the first place, you could run a PCR using primers that target for sequences specific to DNA. There are several answers in this thread that suggest possible tests depending on what kind of DNA may be the contaminant in your specific preps.

https://www.researchgate.net/post/Can_there_be_genomic_DNA_contamination_in_an_RNA_sample

It seems that many people recommend the use of DNase I to help remove DNA contamination. There are some protocols listed in the following link.

https://www.researchgate.net/post/How_can_I_avoid_genomic_DNA_contamination_in_RNA_extraction_using_Trizol_method

Hi,

We frequently isolate RNA from brain tissue using the Qiagen RNA isolation kits (specifically the lipid tissue kit, using Trizol). We have never had problems with DNA contamination, and I believe there is an option to use a DNase with the kit in order to eliminate the contamination if you find it present. Like Sarah said, if it is just a fear of contamination you can always run a PCR for a genomic DNA marker using your isolated RNA to see if the contamination is present.

Hope this helps!

We treat our samples with a turbo DNase then we always do a pcr with universal 16S rRNA gene primers to test for genomic DNA contamination. (I work with bacteria)

We haven't had issues with DNA contamination.

You will almost certainly have some DNA contamination when extracting RNA. A small amount isn't something to worry about. Do a PCR on your sample using DNA primers for an appropriate reference/housekeeping gene to see if there is enough DNA in the sample to warrant further treatment.

I use the Turbo DNA-free kit to clean my RNA samples from DNA contamination. I also like to extract my RNA using the Promega SV Total RNA isolation kit - which has an on-column DNase treatment (Though I still often have to subsequently clean it further with the Turbo kit).

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