Sarah K Frail

3 Questions 2 Answers 0 Followers

Questions related from Sarah K Frail

How to remove or prevent excessive bubbles in semi-solid media?

When I re-melt and stir 0.7% semi-solid media it incorporates a lot of bubbles and the media gets cloudy. It makes it difficult to see the results of phage titer experiments and may be disrupting...

21 April 2017 9,679 3 View

What is standard procedure for making bacteria standard curve using OD and CFU/mL?

My team and I have been doing: 1) Create liquid overnight culture in BHI with single colony of L. monocytogenes 2) Take OD of the sample and record 3) Perform serial dilutions down to 10^-10 and...

02 March 2017 6,274 7 View

Troubles with Titer: How to get good plaques?

My team and I have ordered bacteriophages specific to L. monocytogenes from ATCC and have been attempting to find the titer of them for months now with little to no success. We are unsure if it is...

02 March 2017 3,765 4 View