Is there a simple extraction process for both DNA of a bacteria and RNA of a virus from infected tissue sample without losing either DNA or RNA from the sample ???
Ashwini Shettigar Trizol can be used to isolate RNA, and also DNA at the same time. See attachment.
" TRIzol™ Reagent allows to perform sequential precipitation of RNA, DNA, and proteins from a single sample (Chomczynski, 1993). After homogenizing the sample with TRIzol™ Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interphase, and a red lower organic layer (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the interphase/organic layer with ethanol. "
Trizol is a phase separation extraction method that allows you to extract both DNA and RNA according to the phase you are taking. There are a lot of generic brands with economic versions of similar products.
Adding to the above answers, if you would like to use a commercial kit, then geneOmbio Technologies Pvt. Ltd., Pune, India, has recently optimised viral RNA extraction procedure and developed a kit for viral RNA extraction which is very fast as it takes only 30 minutes for the protocol and gives high-quality RNA without any loss of sample.
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