I accidentally used formamide instead of dimethylformamide to make up my X-gal, it resuspended fine, but can I still use it in my blue/white colour colony selection?
I might add that I don't have enough X-gal to re-make the solution in DMF.
Dear Cate,
YES. You can still use formamide.
Formamide (FA) is a constituent of cryoprotectant vitrification mixtures used for cryopreservation of tissues and organs. Formamide is also used as an RNA stabiliser in gel electrophoresis by deionizing RNA. In capillary electrophoresis, it is used for stabilizing (single) strands of denatured DNA. Another use is to add it in sol-gel solutions in order to avoid cracking during sintering.Formamide, in its pure state, has been used as an alternative solvent for the electrostatic self-assembly of polymer nanofilms.[7]
Dimethylformamide (DMF) has very similar physicochemical properties (polarity and etc) as formamide. Therefore, the latter can replace dimethylformamide.
Hoping this will be helpful,
Rafik
Formamide is not the optimum, but just do it. You have no simple choice anyhow, regarding the costs for Xgal. Cheer up! - With a smile - Johannes.
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