How best to thaw plasma samples for an immunoassay (cytokines)?

When I defrost my plasma samples from -80, I do so on ice, slowly. Then I centrifuge the samples. Upon centrifugation, the plasma samples will have a white sticky substance layer at the top, but...

30 July 2020 4,877 2 View

How can I clean glassware from TiO2 nanoparticles?

In my lab we've been synthesizing 10-20 nm TiO2 nanoparticles from Titanium Isopropoxide, and now the glassware, no matter how I clean it with solvents, is marked with a white patina. I could have...

05 May 2019 5,747 10 View

Influenza viruses plaque assay ?

Hi, I want a correct protocol for plaque assay with influenza viruses . I used MDCK CELLS ( 450 000 cell/well ) with different strains and different plates but i didn't see plaque formation but...

17 February 2019 5,686 4 View

Can protein folding affect antibody detection in a western blot?

I have problems with my western blots in that I get no signal. I have tried all the usual changing of ab concentration, different blocking etc but nothing is working. Is it possible that my...

21 October 2018 5,207 6 View

Have anybody here performed an abductive research strategy as a tool for textual interpretation of interviews?

Do you have any concrete suggestes about the methodological tools to handle abduction? I find that sorting an categorizing the data by coding is not satisfactory, and are looking for an...

01 August 2018 3,983 7 View

Is it possible to use regular Tris-Glycine Running buffer (SDS) on a NuPAGE Tris-Acetate 3-8% precast gel?

15 August 2017 8,144 3 View

THP 1 migration

Hi everyone! Do you have any suggestion regarding THP 1 migration assay? How many cells do you plate in a 24 well transwell with 8 um pore size? Incubation time? Thanks!

08 June 2017 6,333 2 View

Protocol for F4/80 IHC on lung samples?

Hi everyone! I am trying to perform IHC for F4/80 (ab6640 abcam) on pfa fixed LUNG samples, but i'm not able to obtain a good staining. Do you know a good protocol? I usually use a concentration...

20 February 2017 2,652 2 View

What is the best method to concentrate PCR product after pooling several samples?

28 June 2015 5,116 11 View

Does anyone know of a good self-report questionnaire for locus of control?

Need it for a friends thesis which sets out to explore who succeeds in getting back to work after having been unemployed for a period of time.

20 December 2013 6,961 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View