Different RNAdatase comparison?

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Can you suggest me an housekeeping gene for microglia?

I am working with microglia SIMA9 and I will perform a qPCR. If you have already worked with them, can you suggest me one?

05 June 2024 4,930 3 View

Is there any automated catwalk Hydraulic Tray - （catwalks）ajustable resource?

...

10 May 2024 8,189 0 View

P21 molecular weight?

I'm working on senescence, and I'm trying to observe senescence markers. I have performed western blots for p21 but it seems that the bands are at a much higher molecular weight than expected. The...

22 March 2024 9,280 2 View

Why iPSC plated for subcloning die after 3 days?

Hi, I am trying to subclone iPSCs by plating 200-300 cells in 6 wells previously coated with geltrex. When I plate them I use 10uM of rock inhibitor and in theory I j keep it until a nice colony...

11 March 2024 1,063 1 View

Is it possible to prepare root samples for SEM when you don't have the critical point dryer equipment?

I have to analyze Eruca sativa roots and leaves. I've seen some methodologies that involve the utilization of the SPI-Dry Critical Point Dryer, however, I don't have access to it. Can I use a Dry...

27 October 2023 5,617 5 View

Can you culture HUVECs with DMEM ?

Hi, I am culturing HUVECs using the Endothelial Growth Media from PromoCell, however is pretty expensive. Does anyone know any alternative? is it possible to use DMEM for culturing HUVECs?

12 September 2023 9,494 1 View

I'm doing research with the plant model Arabidopsis thaliana, and I've been trying to find information about its NPK content. Does anyone know?

09 March 2023 9,822 1 View

Which are the best cytokines to use as markers for the senescence associated secretome phenotype (SASP)?

Hello, I'm working on senescence and I'm inducing it using TNF alpha. I would like to assess the induction of the phenotype looking for the cytokines typical of the secretome. Which cytokines do...

19 September 2022 2,258 3 View

Silver fir needles collection and storage?

Hi, I am going for a field campaign to collect needles of Silver fir species, I would like to have a good DNA quality for NGS and I am looking for the best method to preserve the needles in the...

16 August 2022 6,805 0 View

Problems with chromosome spread in summer?

Good evening, I am working on determining the karyotype of a beetle species and performing an in situ hybridization. I have difficulty obtaining good chromosome spread (especially in the summer...

05 July 2022 1,454 0 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Could you please suggest methods to compare free protein and immobolized protein binding properties?

I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...

05 August 2024 5,311 0 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Where can I find free research instruments for Nursing?

for eg. transition shock scale

01 August 2024 5,998 0 View

Why do open and free science in a world where science is not open and free?

Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.

01 August 2024 10,046 1 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Oscar Gabriel Beltran

You can use

http://ensemblgenomes.org/info/data/ncrna

where there´re some resources mainly:

http://www.mirbase.org/

Camilla Boschian

Thank you!

Bruno César Feltes

Hi Camilla. In which way do you want to compare your datasets? There are numerous tools for gene expression data comparison, but it depends on your goal. Can you elaborate?