different between gene centric approach and assembly approach in omics study ??
how to avoid primer dimer formation ?? im getting primer dimer even after increasing the temperature, increasing DMSO , what should be done ??
01 February 2018 3,453 7 View
I have a 16s rRNA amplicon sequences (next generation sequence), I want to find out the phylogenetic diversity of a region, How can I do that? please tell me the different approaches. thanking...
01 January 1970 9,350 2 View
I have 16s metagenomic raw data to be analysed , please describe me different software and its work application to analyse it with respect to diversity ?
01 January 1970 5,405 3 View
I have to prepare a predictive model on effect of climate change on iceland/ frozen soils, please suggest me a tools and how can i achieve this ?
01 January 1970 3,570 3 View
While doing the PCR amplification of metagenomic DNA, I got stuck with no visible bands on gel. But there is always the presence of primer dimers in the gel. I had added about 2.5ul of DNA...
01 January 1970 7,103 5 View
I was working on plasmid isolation from an organism but i didn't got plasmid via plasmid isolation kit. However, later on i extracted the gDNA from that portion of the given organism, luckily i...
01 January 1970 3,255 5 View
hello everyone , i want to check the diversity of Methanogen of a particular soil , please suggest me any idea or pipeline for this analysis, and also suggest me a particular primer for the...
01 January 1970 5,791 1 View
hello, i want to estimate carbon dioxide and methane from soil , any suggestion and recommendation will be most kind , thanking in anticipation .
01 January 1970 7,775 4 View
I want to design a primer pair to amplify the genomic DNA of a saline water species. Can anyone help me out to design a species-specific primer that not only amplify small fragments but also can...
01 January 1970 4,247 7 View
My primers amplification got many time primer dimer, and i did not get my desired band in pcr amplification. I want to cut the genomic DNA from the gel, and use it as the template for a new PCR...
01 January 1970 9,850 7 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View