Ahmad Ali

12 Questions 6 Answers 0 Followers

Questions related from Ahmad Ali

Different between gene centric approach and assembly approach in omics study ??

different between gene centric approach and assembly approach in omics study ??

11 November 2018 5,636 0 View

How to avoid primer dimer formation ?? im getting primer dimer even after increasing the temperature, increasing DMSO , what should be done ??

how to avoid primer dimer formation ?? im getting primer dimer even after increasing the temperature, increasing DMSO , what should be done ??

02 February 2018 3,411 7 View

How to design a species specific primer ?

I want to design a primer pair to amplify the genomic DNA of a saline water species. Can anyone help me out to design a species-specific primer that not only amplify small fragments but also can...

01 January 1970 4,229 7 View

How do you use gel-extracted DNA (size almost 1500-3000bp) in a new PCR reaction?

My primers amplification got many time primer dimer, and i did not get my desired band in pcr amplification. I want to cut the genomic DNA from the gel, and use it as the template for a new PCR...

01 January 1970 9,828 7 View

What are the best primer for Methanogen amplification ?

hello everyone , i want to check the diversity of Methanogen of a particular soil , please suggest me any idea or pipeline for this analysis, and also suggest me a particular primer for the...

01 January 1970 5,773 1 View

What are the important data, parameters and software needed to predict a model the check the impact of climate change in a particular region?

what are the important data and parameters needed to predict a model to check the impact of climate change on a region? please suggest me your ideas to overcome this problem .

01 January 1970 9,644 5 View

What is the best software to prepare a predictive model on climate change on normal 64 bit laptop ?

I have to prepare a predictive model on effect of climate change on iceland/ frozen soils, please suggest me a tools and how can i achieve this ?

01 January 1970 3,554 3 View

Which bioinformatic tool is feasible to explain 16s metagenomic data ?

I have 16s metagenomic raw data to be analysed , please describe me different software and its work application to analyse it with respect to diversity ?

01 January 1970 5,385 3 View

What are the various methods to estimate Co2 and Methane emission from soil? what are the equpiments/instruments through we can measure the emission?

hello, i want to estimate carbon dioxide and methane from soil , any suggestion and recommendation will be most kind , thanking in anticipation .

01 January 1970 7,755 4 View

Does anybody know how to extract Plasmid DNA from isolated genomic DNA.

I was working on plasmid isolation from an organism but i didn't got plasmid via plasmid isolation kit. However, later on i extracted the gDNA from that portion of the given organism, luckily i...

01 January 1970 3,234 5 View

What is the reason for Primer Dimer formation in a PCR reaction? Does PCR reagents have any role in Primer Dimer formation?

While doing the PCR amplification of metagenomic DNA, I got stuck with no visible bands on gel. But there is always the presence of primer dimers in the gel. I had added about 2.5ul of DNA...

01 January 1970 7,084 5 View

How can I access the Phylogenetic diversity of a region using 16s rRNA amplicon sequences?

I have a 16s rRNA amplicon sequences (next generation sequence), I want to find out the phylogenetic diversity of a region, How can I do that? please tell me the different approaches. thanking...

01 January 1970 9,333 2 View