What are the best primer for Methanogen amplification ?

01 January 1970 1 6K Report

hello everyone , i want to check the diversity of Methanogen of a particular soil , please suggest me any idea or pipeline for this analysis, and also suggest me a particular primer for the amplification of methanogen.

Abhijeet Singh

16S primers for Archaea or mcrA primers.

Badges
Science method

More Ahmad Ali's questions See All

Different between gene centric approach and assembly approach in omics study ??

different between gene centric approach and assembly approach in omics study ??

10 November 2018 5,660 0 View

How to avoid primer dimer formation ?? im getting primer dimer even after increasing the temperature, increasing DMSO , what should be done ??

how to avoid primer dimer formation ?? im getting primer dimer even after increasing the temperature, increasing DMSO , what should be done ??

01 February 2018 3,453 7 View

How can I access the Phylogenetic diversity of a region using 16s rRNA amplicon sequences?

I have a 16s rRNA amplicon sequences (next generation sequence), I want to find out the phylogenetic diversity of a region, How can I do that? please tell me the different approaches. thanking...

01 January 1970 9,350 2 View

Which bioinformatic tool is feasible to explain 16s metagenomic data ?

I have 16s metagenomic raw data to be analysed , please describe me different software and its work application to analyse it with respect to diversity ?

01 January 1970 5,405 3 View

What is the best software to prepare a predictive model on climate change on normal 64 bit laptop ?

I have to prepare a predictive model on effect of climate change on iceland/ frozen soils, please suggest me a tools and how can i achieve this ?

01 January 1970 3,570 3 View

What is the reason for Primer Dimer formation in a PCR reaction? Does PCR reagents have any role in Primer Dimer formation?

While doing the PCR amplification of metagenomic DNA, I got stuck with no visible bands on gel. But there is always the presence of primer dimers in the gel. I had added about 2.5ul of DNA...

01 January 1970 7,103 5 View

Does anybody know how to extract Plasmid DNA from isolated genomic DNA.

I was working on plasmid isolation from an organism but i didn't got plasmid via plasmid isolation kit. However, later on i extracted the gDNA from that portion of the given organism, luckily i...

01 January 1970 3,255 5 View

What are the various methods to estimate Co2 and Methane emission from soil? what are the equpiments/instruments through we can measure the emission?

hello, i want to estimate carbon dioxide and methane from soil , any suggestion and recommendation will be most kind , thanking in anticipation .

01 January 1970 7,775 4 View

How to design a species specific primer ?

I want to design a primer pair to amplify the genomic DNA of a saline water species. Can anyone help me out to design a species-specific primer that not only amplify small fragments but also can...

01 January 1970 4,247 7 View

How do you use gel-extracted DNA (size almost 1500-3000bp) in a new PCR reaction?

My primers amplification got many time primer dimer, and i did not get my desired band in pcr amplification. I want to cut the genomic DNA from the gel, and use it as the template for a new PCR...

01 January 1970 9,850 7 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

How do microbial communities contribute to ecological cycles on Earth and how does an agroecological system support diverse microbial communities?

25 July 2024 5,197 2 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View