hello everyone , i want to check the diversity of Methanogen of a particular soil , please suggest me any idea or pipeline for this analysis, and also suggest me a particular primer for the amplification of methanogen.
16S primers for Archaea or mcrA primers.
different between gene centric approach and assembly approach in omics study ??
10 November 2018 5,563 0 View
how to avoid primer dimer formation ?? im getting primer dimer even after increasing the temperature, increasing DMSO , what should be done ??
01 February 2018 3,327 7 View
I have a 16s rRNA amplicon sequences (next generation sequence), I want to find out the phylogenetic diversity of a region, How can I do that? please tell me the different approaches. thanking...
01 January 1970 9,267 2 View
While doing the PCR amplification of metagenomic DNA, I got stuck with no visible bands on gel. But there is always the presence of primer dimers in the gel. I had added about 2.5ul of DNA...
01 January 1970 7,031 5 View
I have 16s metagenomic raw data to be analysed , please describe me different software and its work application to analyse it with respect to diversity ?
01 January 1970 5,329 3 View
I have to prepare a predictive model on effect of climate change on iceland/ frozen soils, please suggest me a tools and how can i achieve this ?
01 January 1970 3,501 3 View
I was working on plasmid isolation from an organism but i didn't got plasmid via plasmid isolation kit. However, later on i extracted the gDNA from that portion of the given organism, luckily i...
01 January 1970 3,181 5 View
hello, i want to estimate carbon dioxide and methane from soil , any suggestion and recommendation will be most kind , thanking in anticipation .
01 January 1970 7,701 4 View
My primers amplification got many time primer dimer, and i did not get my desired band in pcr amplification. I want to cut the genomic DNA from the gel, and use it as the template for a new PCR...
01 January 1970 9,775 7 View
I want to design a primer pair to amplify the genomic DNA of a saline water species. Can anyone help me out to design a species-specific primer that not only amplify small fragments but also can...
01 January 1970 4,176 7 View
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...
01 March 2021 360 7 View
I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...
28 February 2021 606 3 View
hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?
28 February 2021 1,254 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...
26 February 2021 3,033 9 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View