Hello everyone, can someone explains about the differences between HIV-1 with NCBI Reference Sequence: NC_001802.1NCBI and HBX2 GenBank: K03455.1?
Thank you.
Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...
24 February 2021 9,105 2 View
In our discipline (chemistry), annual meetings of the American Chemical Society (ACS) used to be mega-events with up to 20,000 participants. The current one will be virtual, and the organizers...
06 January 2021 1,882 5 View
Despite certain disadvantages, peer-review is generally accepted as a quality control of manuscripts submitted to scientific journals. The higher the rank of the journal, the more often...
03 December 2020 3,235 17 View
Citations of research papers are the topic of various threads on this platform. Quite often researchers ask how to increase the number of citations. I did my first 30 years of chemical research in...
01 December 2020 6,173 94 View
LSTM has two hidden layers, I want to know the number of cells in each hidden layer.
30 November 2020 3,481 4 View
Chemistry is frequently termed the "central science". It underlies the foundation of modern civilization (drugs, plastics, textiles, dyes, fertilizers etc.), but the public perception of chemistry...
30 November 2020 1,113 15 View
Chemistry is frequently termed the "central science". As such, chemistry is expected to play a major role in fighting the coronavirus. For example, hand sanitizers and drugs are all based on...
30 November 2020 4,983 17 View
As dedicated researchers we all try to contribute to the advancement of our discipline. Often the work can be routine, but occasionally there are some real highlights. Can you please share your...
23 November 2020 4,150 20 View
Please provide your opinion on vertical farming. Is it the future of agriculture? If yes, where is it most promising (areas, countries cities etc.)?
17 November 2020 3,934 97 View
Most researchers have made individual experiences with publishing in peer-reviewed journals. What is your opinion about the peer-reviewing process in general? In your opinion, is it "censorship",...
28 October 2020 3,631 97 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?
21 February 2021 7,783 12 View
Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...
11 February 2021 562 6 View
I'm looking for the genetic sequence for the TAR region of HIV. If I put Transactivator Response Region, and HIV, and "genetic sequence: into a browser...is it not unreasonable to expect to be...
10 February 2021 658 5 View
Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...
21 January 2021 2,593 1 View
Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...
05 January 2021 4,214 3 View
I have annotated a genome successfully with PROKKA. I can upload the genome to NCBI, but I cannot upload the annotation. PROKKA does not produce files in the correct format, and I cannot find any...
29 December 2020 3,976 3 View
I am working with THP-1 cells at the moment and am having trouble with maintaining a high confluence once I seed them on a 24-well plate. I usually try to seed around 100,000 cells per well and...
29 December 2020 1,567 3 View
How to use NCBI to design primers for specific Micro RNAs for quantitative analysis and how to check the efficacy of the selected primer before working
24 December 2020 180 3 View
Right now, I want to design qPCR primer for my target gene. But that gene has 21 predicted mRNA sequence in the NCBI database. Most of the sequence names are same but only different location...
23 December 2020 5,531 12 View