Hello everyone, can someone explains about the differences between HIV-1 with NCBI Reference Sequence: NC_001802.1NCBI and HBX2 GenBank: K03455.1?
Thank you.
SMEs are backbone of the country's white economy, most of them are II generation entrepreneurs and migrated from family owned business policies, and having sufficient higher educations for...
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Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View
A stem cell can divide to produce 2 identical copies of itself, which is called self-renewal. Then what is proliferation in terms of stem cells. Or is this term 'proliferation' applicable to stem...
04 June 2024 6,245 4 View
We have transfected the plasmid with lipofectamine, the plasmid will eventually move into the nucleus for transcription. Does this plasmid remain in the nucleus during cell replication? if so how...
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16 May 2024 5,106 0 View
I have to rear the R-strain for my research
13 May 2024 3,394 0 View
In Scopus, we can check the paper indexed in Scopus or nor? I do not know how to check the paper indexing in SCI and SCIE.
09 April 2024 906 2 View
I recently performed Illumina shotgun sequencing and my fastqc data is as attached. is this correct?
01 April 2024 3,354 6 View
I just failed about my PhD application (Business field). I think one of the reasons is "No Publication". But I have limited access of academic journals (usually use Google Scholar) and no...
21 March 2024 8,988 4 View
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I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...
20 July 2024 1,339 2 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
Hello, I was attempting to download some sequences data from NCBI. The format "gene features" could help me extract certain gene fractions using the names, but I found that it seems to be...
04 July 2024 9,875 1 View
Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...
03 July 2024 3,884 5 View
I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...
02 July 2024 5,332 3 View
We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...
01 July 2024 7,644 3 View
I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...
30 June 2024 6,502 5 View
Dear Sir, I hope this message finds you well and in good health. I am writing to seek your guidance regarding an issue I encountered during the annotation of my phage sequence using DNA Master....
30 June 2024 8,650 0 View