Hi folks,

I'm trying to identify the intermediates produced from a degradation of Sodium Diclofenac. I'm using a QTOF (Agilent) and the method in negative and positive mode is from Agilent but for LC-QQQ). I have tried the positive mode and the TIC chromatogram is different from the UV but I get one peak for my standard sample (10 ppm) and 3 peaks for the degraded sample. However, I cannot identify the products yet (the results are inconsistent).Then I tried the negative mode, and the TIC and UV chromatogram are the same (great).However, I get 2 peaks for the standard (10 ppm) where one of them is broader.And for the degraded sample I get overlapped peaks. I increased the concentration of the standard to 50 ppm then I got 1 peak. I tried to optimize the system with different gradient, collision energy, fragmentor voltage but no luck.

Does anyone has any idea of what is wrong, please?

Why when I'm at the negative mode I see 2 peaks for SD 10 ppm and at positive 1 peak for SD 10 ppm but no consistency with TIC and UV?

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