I am fairly new to cell cultivation and I am wondering about the main differences between the FreeStyle 293 media from Gibco and the Pro293-DM from Lonza. As I understand it both are serum and protein free chemically defined medium intended for cultivation of HEK-293 cells. The only difference I can see is that the Pro293 requires adition of L-Glutamine. Is this the only practical difference? Do both work just as well for this and for use in transitient expression of proteins?
If anyone has experience of comparing the two I would be delighted if you would share it with me.
Hi Anette,
I found two differences by reading the MDS:
FreeStyle 293 media from Gibco: has stable Glutamine (=Glutamax) and it is The medium is not recommended for adherent 293 .
Pro293-DM from Lonza: is without Glutamine and Phenol red
I`m wondering about this Pluronic® in the Lonza Medium. I`ve never hesred abbout this and for me the reason of usng this is not clear.
Which Medium you choose is depending of what you wnt to do. If you want to transfect the cells and check if they are expressing the protein yoou can do this in a much easier and cheaper way which also works for stable clones.
I`m not experienced in doing real large scale production of proteins, so therefor I can only help you with the "normal" way to transfect HEK 293 cells.
Good luck
Sibylle
Following up on Sibylle's comments, media with glutamax is very stable and will have a long shelf life (up to a year), while media with L-glutamine is only good for about 1 month after addition of L-glutamine since the glutamine breaks down into ammonia (= toxic to cells). But the shelf life depends how much L-glutamine you have to add (more = more ammonia).
Pluroinc is a surfactant in case your going to grow the cells in suspension.
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