Why IPNI voluntarily abbreviates some people's surnames in their standard forms?

The International Plant Name Index is known for standardising plant scientific names, including the standard forms of authors' names. I heard that one person's standard form may be chosen when...

01 January 2021 1,928 5 View

Which variables are related to transportation infrastructure?

I am conducting research on the "Impact of Transport Infrastructure on Economic Growth" Dependent Variable: GDP Growth (World Bank) Independent Variable : Railways, goods transported (million...

18 November 2020 9,014 6 View

How do I enter multiple points of I-V MOV characteristic from an external file in PSCAD?

I have a multiple points from a I-V MOV characteristic from a equipment datasheet, but PSCAD only allows to enter eleven points. I saw there is an option to enter until 100 points from an external...

28 February 2020 2,737 3 View

MOV modelling using PSCAD?

What are the main parameters that should be entered in pscad for MOV modelling? The MOV will be part of the overvoltage protection circuit of a series capacitor in a transmission line. And how do...

27 February 2020 2,055 2 View

How to codify a sequence DNA correctly for DNA barcoding analysis?

Hello, I am starting a new project with DNA barcoding and I am not sure how to codify sequence DNA. In this project we are using the following molecular markers: rps16-trnk, rpl32-trnl and...

26 July 2018 5,797 2 View

How to create interaction plots of 2x2x2 mixed ANOVA (BWW Design) in R?

My current study looks at how Chinese and English view different advertisements that were manipulated on the length and type as...

10 May 2018 263 3 View

No title

31 October 2017 867 1 View

Where is the "Boca de la Raspadura" in Colombia?

24 August 2016 8,571 3 View

Can trypsin affect the cellular receptor to dengue virus infection?

The trypsin can change the VERO receptors used by dengue virus infection.

17 August 2016 1,879 3 View

How can I isolate Canine Dispar Virus (CDV) of dog plasm?

02 August 2016 2,327 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View