Hi Wasim,

The LAD ligation technique is a well established model, yields reproducible rat model for MI and cardiac ischemia. The trick to succeeding in bringing post-operative mortality rate to a min, is a good surgical skills, a volume controlled Rodent Ventilator and, preferably, a surgical microscope.

Check this article:

Article Establishment of a rat model of myocardial infarction with a...

what I did differently is applying a figure of 8 suture around the artery capturing a pit of the heart muscle thickness as the suture tends to cut through the artery .

Please do not hesitate to ask follow-ups.

Best of Luck,

Ghanim.

Sir, thank you very much for help. I vl b in touch with you n vl let you know about the progress.

Regards