Hello colleagues,
This is one of my first IPs so I am a bit confused with the result I just obtained.
I used the dynabeads protein G for my purposes.
Here it is, my protein is ~97kDa (in the red box) and it seems that I have it in the IP, however when I screen that IP's supernatant, negative control supernatant (that I IP'd with irrelevant IgG) and just the cell lysate I see almost at the same level (but a little higher) the protein that I am actually trying to IP ...
So here I am not sure that what's been IP'd is actually something that may interest me. (it is interesting but maybe it's just not an IP signal)
Shouldn't the IP and control signals be at the same level?
Or it is a normal situation? If yes, why that happens?
Thank you!