Clement M Lee

2 Questions 7 Answers 0 Followers

Questions related from Clement M Lee

Any ways to boost exogenous protein levels in COS7, H1299 or HeLa cells?

I transfected COS7, H1299 and HeLa cells with an expression plasmid (pCMV-Tag2B) for an exogenous protein. The exogenous protein levels were low. Are there any ways to boost the expression levels...

01 January 2018 7,787 6 View

Are there any good dyes for 3-color immunofluorescence imaging?

I would like to perform 3-color immunofluorescence imaging on three proteins in cells. One of them is EGFP-tagged. The red color dyes (Texas Red or Alexa Flour 568) worked very well. However, the...

12 December 2014 2,334 6 View