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I plan to use Lipofectamin 2000 to transfect human macrophage in vitro. Macrophage is not a easy target for tranfection and there are not many papers on tihs topic. Does someone has some...
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I tried to use irradiated pig PBMC to stimulate human PBMC. The proliferation seems low (8%-20%). Is it normal? Has anyone had any experience?
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Even when I use the same cell, same voltage, same ab concentration, the position of the population tend to fluctuate in different samples(and often much higher than unstain). I personally think it...
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