Yixiong Tan

5 Questions 8 Answers 0 Followers

Questions related from Yixiong Tan

Should hypoxia induce or inhibit reactive oxygen species (ROS)?

Since ROS is a byproduct of oxygen metabolism, it is reasonable to think that hypoxia can inhibit ROS by reducing O2. But hypoxia also causes many pathological changes (such as apoptosis) via...

03 November 2017 8,559 4 View

Did anyone use Lipofectamin 2000 to transfect human macrophage before?

I plan to use Lipofectamin 2000 to transfect human macrophage in vitro. Macrophage is not a easy target for tranfection and there are not many papers on tihs topic. Does someone has some...

13 January 2017 9,898 4 View

Has anyone used irradiated pig PBMC to stimulate human PBMC?

I tried to use irradiated pig PBMC to stimulate human PBMC. The proliferation seems low (8%-20%). Is it normal? Has anyone had any experience?

16 September 2015 8,431 2 View

Has anyone done intracellular staining of attached cells for flow cytometry before?

I want to detect IL-10 expression of macrophages. Is it possible to apply Golgistop(4h) and permeabilization to the cells after using enzyme to detached them from the flask?

16 December 2014 3,249 3 View

Does anyone have any experience with population position change in flow cytometry?

Even when I use the same cell, same voltage, same ab concentration, the position of the population tend to fluctuate in different samples(and often much higher than unstain). I personally think it...

20 September 2014 8,146 13 View