Hi Asmaa

I didn't have experience in Turbofect, but the Lipogene transfection reagent can transfect MEFs with a good efficiency, you could have a try, here is a web link: www.genemedi.net/i/lipogene-transfection-reagent

Hi Asmaa

I didn't have experience in Turbofect, but the Lipogene transfection reagent can transfect MEFs with a good efficiency, you could have a try, here is a web link: www.genemedi.net/i/lipogene-transfection-reagent

see here use of Viromer RED for plasmid transfection in MEFs (suppl Fig S2)

Article Tumor suppressor BTG1 promotes PRMT1-mediated ATF4 function ...

Thanks alot for your suggestions. There are many lipid-based products for transfections of MEFs but conflicting data, do you think lentivirus would be an option for transfecting the cells with luciferase reporter ?