Did anyone try using Neurobasal-A with embryonic neurons?

19 December 2015 1 9K Report

Dear all,

Did anyone try using Neurobasal-A with embryonic cultures (preferably mouse) and were there any problems or a noticeable difference when compared to Neurobasal?

I have Neurobasal-A in stock and I know that Neurobasal worls fine with adult neurons, so I wonder if I can just use Neurobasal-A for embyoinic cultures.

Cheers,

Michal

Utkarsh Kapoor

Hi Michal,

I regularly use Neurobasal -A medium to generate primary neurons from mouse embryos. Frankly speaking, I have never compared Neurobasal vs Neurobasal-A in parallel.

Even though their technical manual says that Neurobasal -A medium is for adult neurons, I have used it with B27 supplement very routinely without facing any problems.

Hope this helps. Good luck!

Utkarsh

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