Problem: we want to know - at the END of an experiment - if a signalling pathway was activated DURING the experiment.
I had read something about a novel technique that allowed this. I think that CRISPR-based genome editing was used to leave traces in the genome that were used to detect the pathway activation. Unfortunately, I thought at the time that this was interesting but did have a use for it and forgot the title etc. Now I cannot find the paper anymore - no matter how I google.
Does anyone know the method and could point me to the paper? Or are there any other such methods? Our preferred target would be mouse hepatocytes - not cell culture.
Cre/LoxP systems could conceivably do this. Put your Cre under control of a promoter activated by the signalling pathway, include a marker (betaGal etc) with a floxed suppression cassette. If your pathway is activated, cre is expressed, the cassette is cut out and you get betaGal in any cell that had activation.
Dear John, thanks for the hint. This sounds like a solution but one caveat is that it will only work with on/off types of pathways and will be recording activity from day one. Perhaps one could combine it with tamoxifen dependent cre to restrict it time-wise. Will search for this now, probably it's the system i read about.
With the right keywords I found an article about such a system - Article Expression of Cre recombinase as a reporter of signal transd...
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