Does anyone know what the wavelength for the detection of oleuropein under UV detector of HPLC device is ?
The main chromophore is a benzine ring functionalized with phenols.
This suggests that 254 and 280 nm are useful
Actual maxima are 228.5 and 278 nm- as per reference below
(https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0ahUKEwj1uaif-8DYAhXJ0iYKHXF6BDAQFggnMAA&url=https%3A%2F%2Fwww.researchgate.net%2Fprofile%2FMaria_Clodoveo%2Fpost%2FDoes_anyone_know_anything_about_olive_oil_nutraceuticals%2Fattachment%2F59d62969c49f478072e9c3cc%2FAS%3A272469666009089%401441973238928%2Fdownload%2FBiochemical%2Band%2BBiological%2BStudies%2Bon%2BOleuropein%2Band%2BIts%2BHypoglycemic%2BEffect.pdf&usg=AOvVaw2feqR6qVTQ_I70R05_yzMX)
Method development of similar compounds is best done using a UV/VIS diode array detector ("DAD" or "PDA") so you can scan all wavelengths, all the time, to aid you in identifying the sample and also, "non"-sample peaks. *A quick keyword search on the web shows a std wavelength of 280 nm being used so that would be a good starting point.
BTW: If you have a pure standard of any material, just put some in a cuvette and run an analysis using a spectrophotometer (an instrument which is found in any modern laboratory). That is one of the best and quickest ways to find out which wavelengths could be used for any pure sample BEFORE you start an HPLC analysis. If the amount of material is very small, there is another way to accomplish the same thing...
- "Method Development Hint: Use your HPLC Diode Array Detector (DAD or PDA) as a Spectrophotometer": https://hplctips.blogspot.com/2013/09/method-development-hint-use-your-hplc.html
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