DSSP latest version?

Good day, I have downloaded the latest version of DSSP from the site : https://swift.cmbi.umcn.nl/gv/dssp/ however as I downloaded and extract the package I see it is named hssp 1.3.4 and the...

31 December 2019 3,619 1 View

Error in relaxation Sc2CF2 VASP ?

11 December 2019 8,549 3 View

Cellulose topology file?

08 September 2019 4,111 3 View

Cellulose in Gromacs?

Hello, I have seen some papers simulating cellulose in GROMACS software. I have not tried the simulation of non-protein or DNA molecules in GROMACS and want to know how should I use cellulose in...

05 June 2019 4,642 3 View

Raman spectra of GO/nZVI/AC nanocomposite?

Hello, I have taken raman spectra from my nanocomposite comprising Active carbon , graphene oxide and nano zero-valent iron (nZVI). The concentration of nZVI is fixed in all the samples attached...

04 May 2019 7,445 3 View

Nanofibers vs nanotubes?

Hello, Can somebody suggest a good paper or website discussing the characteristics and differences between nanotubular and nanofiber structures and their applications. Herein, I am not looking...

02 March 2019 1,392 2 View

Graphene oxide Formation with respect to the formed peaks in raman spectra?

According to my raman spectra I am not really sure what the two peaks demonstrate and here graphene oxide is reduced by sodium borohydride. I appreciate if somebody can give me a hint.

07 August 2018 4,981 5 View

Three-valent arsenic solvation in distilled water ?

Why can't three-valent Arsenic be solved in distilled water and is there a solution to make it soluble in distilled water ? Thanks.

05 June 2018 8,062 1 View

How to obtain Cellulose nanocrystals in the nano scale?

04 May 2018 6,447 3 View

GROMACS: solvent addition ?

I wonder if it is possible to add a buffer (like tris) as the solvent in GROMACS package rather than water molecules by using : gmx solvate and the -cs command

04 May 2018 398 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View