Good day Mr. Mwesigwa! I found this helpful if not on late reply to you.

(1)On Sample Preparation, harvest the infected cells and extract DNA from them using a DNA extraction kit. (2) then employ PCR to amplify the HBV cDNA. Design primers specific to the HBV genome.

(3) Create a standard curve using known concentrations of HBV DNA or plasmids containing HBV DNA. This will help in quantifying the unknown samples.

(4) Perform real-time PCR to amplify the cDNA in your samples. Monitor fluorescence during each cycle to track DNA amplification in real-time. Use the standard curve to quantify the amount of HBV cDNA in your samples based on the cycle threshold (Ct) values obtained in real-time PCR Normalize the HBV cDNA quantity to a reference gene or an internal control to account for variations in sample preparation. (5) Analyze your results to determine the HBV cDNA levels in the infected cell cultures. (6)Validate your findings through replicate experiments and controls to ensure accuracy and reproducibility.