Detected Sb in Sn/Ni plating surface under EDX mapping and also spot. Any explanation this could happen? Possible that electroplating chemical bath contained Sb?
Hi everyone! After thinking about it on my own, I'm posting a question here. Has anyone ever tried creating qPCR standard curve samples (plasmid or DNA standard) by inserting multiple target...
30 June 2024 1,569 1 View
How to purify boron esters? Hello. I'm synthesizing borates in my lab. My questions are as follows: 1. How to purify boronate esters like B(OR)3 compounds, and it is a UV undetected...
23 May 2024 7,072 2 View
We are currently doing community work where we have to measure the height and weight of children 6-10 years old. The community work is done across different communities. Thus, since the...
03 April 2024 7,311 0 View
Hi All, I would like to seek for your expert input for below queries on RNA QC for RNASeq: Can we proceed for RNASeq run on RNA less than 10ng, with DV200 in between 68 - 80%?Details as below:...
18 March 2024 5,936 2 View
I am currently conducting research on purifying Myoviridae phage particles using sucrose density gradient ultracentrifugation. However, I have encountered a challenge where no visible opalescent...
12 March 2024 9,387 3 View
hi I just start building a nonmem pharmacokinetics model. i got a problem with my estimate result .. is there any reason for %RSE=0?? if anyone know about this issue , please explain this...
04 March 2024 6,077 0 View
I'm following a protocol to bind fluorescent ligand to receptors expressed on cells in a microplate. The protocol includes fixing cells with 4% PFA for 20 mins. This prevents cell loss during...
09 January 2024 7,517 7 View
Looking for somebody who had similar experience when you use DOI issued by ResearchGate. I published one paper in a conference in 2007. The conference seems not to publish their proceeding, and I...
25 December 2023 5,537 1 View
Required a research paper with raw data to conduct a comparison of recent research and future direction
05 December 2023 2,528 1 View
Could I obtain a compact film through the atomic layer deposition method? If yes, why as-deposited SnO2 film with a thickness of 17 nm fail to protect the underneath layer film of my device? Could...
05 November 2023 8,153 0 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 947 1 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
I am simulating interlocking / dry stacked masonry in Abaqus. I am using a friction coefficient of 0.6 for my model as it is stated in the previous research as well as in the base paper. The...
16 July 2024 5,047 0 View
Aim is to simulate the shape of Mobius strip (an elastic ribbon) in simple way ( using Abaqus Learning edition). In literature, Mobius strip is modeled as Kirchhoff Rod / Cosserat plate. But, such...
15 July 2024 4,388 1 View