I am working on new viruses and i have encounter a problem. After complete sequencing, i came to know during analysis that Rdrp do not have the conserved regions. In the sequence if G replace with A, which will make that base pairs a start codon, then conserved regions will be fulfilled like the reference virus. But problem is that i have tried so many times and sent 35 clones to company to check the sequence of bases at that region but results indicates it have G at that place. So, i need suggestions regarding that.