Hello,

Could you tell us if this is from human or murine brain? Is your tissue from old animals?

The resolution is not very good but in some neurons you can see a punctate pattern. If this is from old mice, this could be autofluorescence from lipofuscin. A good way to tell if this is the case is to check for the same autofluorescence in other channels. You can remove most of this autoflluorscence using TrueBlack.

These are murine brains, some are old depending on the cohort. I can see the stain is in neurons, but do you know why the stain occurs intracellularly rather than extracellular along the axons where MBP typically is, or can you recommend any paper that has studied this before in the CNS?

Hello again,

Please check my previous answer. I think that this is not real staining but a tissue artifact present in brains of older mice. Consider the use of TrueBlack to remove this artifact.

Hi,

Thanks for your reply. I ask because previous work has shown the stain to be intracellular through phagocytosis, shown by lysosomal staining (like LAMP2, shown in this paper - Article TREM2-Transduced Myeloid Precursors Mediate Nervous Tissue D...

Hello,

By their shape, these are clearly neuronal cells and not microglia or infiltrating phagocytes.

Good luck