Rae-Ling Lee

5 Questions 6 Answers 0 Followers

Questions related from Rae-Ling Lee

Best staining protocol for live neuron culture calcium staining?

I am working on a protocol to measure calcium flux by synaptic signaling in live mouse neuron culture. I am using Fluo-4 AM for my stain, which I have seen others use, but it is not showing any...

01 February 2023 7,291 4 View

Fluo-4 AM signal decay?

Hi all, I'm staining live neurons with Fluo-4 AM esters (Thermofisher cat# F14201) and seem to be having some issues when capturing images. With video recording with continuous excitation, there...

03 November 2022 2,520 0 View

Intro to R

I'm in the throes of learning R and am working with the book "Getting Started with R" by Beckerman and Petchey. Does anyone have any suggestions for other resources that are less "fluffy" and have...

01 January 1970 2,638 9 View

Tips on preventing contamination of mouse microglial cultures?

I'm having trouble with bacterial contamination of microglial cultures - dissection is done under a hood and all buffers and reagents have been checked for contamination. (Funny thing, the first...

01 January 1970 2,990 2 View

Degraded myelin basic protein in the CNS

Hi all, I’m staining murine brains for degraded myelin basic protein (sometimes called cryptic MBP, also known as MBP69-86) and would like input on what we are seeing. Basically, it doesn’t look...

01 January 1970 6,518 5 View