How to extract RNA from brown adipose tissue(BAT)?

30 January 2021 6,610 3 View

How can we test interaction effect using repeated measure design?

Hi! I am currently working on a problem using repeated measure design. Each participant was assigned to two CONDITIONs (A and B). And their GOAL (continuous) were measured twice in condition A and...

25 November 2020 1,235 3 View

When performing mutliple behavioural tests over a period of time, should you habituate the animals before every test or just once at the beginning?

I am doing a baseline + 2 rounds of behavioural testing (NOR + NLR) - so total 3 rounds of testing, all 1 week apart in male SD rats. Is it necessary to habituate them before every test, or is...

17 June 2020 9,083 5 View

Can I use LS column and MidiMACS seperator from Miltenyi Biotec Neutrophil Isolation Kit for mouse peripheral blood?

We are using the neutrophil isolation kit from Miltenyi Biotec to get the neutrophils from peripheral blood from JAK2 constitutively active mice. This mouse is a leukemia model, so the traditional...

11 March 2020 8,611 3 View

Can I use satellite images to detect the leaking and clogging for a Surface Drip Irrigation System?

Hello everyone: I have being searching for three weeks now to find a possible solution using remote sensing. This application is for a vineyard 200m by 300m or even bigger. It has a a surface...

28 February 2020 3,723 5 View

Is it normal that Streptomyces fradiae culture is clumpy/particles/precipitated?

Hello, I cultured Streptomyces fradiae from a freeze-dried sample following ATCC protocol: use 6ml broth (tryptone 5g/l, yeast extract 3g/l) to rehydrate the sample and culture at 26 degrees...

05 February 2020 1,232 3 View

How to check the quality of MgO for magnetic tunnel junction?

I am making some magnetic tunnel junctions, but I always get the junction shorted. I am not sure whether the quality of MgO is good. Does anyone know how to check the quality of MgO? Such as AFM...

28 January 2020 139 2 View

Implement Topshim (Bruker NMR) shimming commands in IconNMR?

With Bruker's Topshim program, I have used the following command to shim every new sample: topshim z6 1d optsolvent coil=1H tuneb tunea Now I'd like to use IconNMR to automate this procedure,...

20 August 2019 1,111 5 View

How to dissolve and inject 4-hydroxytamoxifen to nude mouse?

What is the normal way to inject 500 ug 4-hydroxytamoxifen per mouse? Is it dissolved 4-OHT in ethanol and diluted with peanut oil at least 10 times?

10 May 2019 3,301 1 View

How to mesh in crack tip using shell element in ABAQUS?

S8R elements were applied in 2D cracked plate in ABAQUS. But when I drew a circle contour in the crack tip, it showed an error. I also checked the ANAQUS Manual and it told me that 'Focused...

21 April 2019 3,698 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How to show arrows over the streamlines in CFD-post?

I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...

01 March 2021 8,668 3 View